Editing CrocoBLAST:UserManual (section)

=2. Hardware requirements=
Because of the nature of the data being processed, it is better if your computer has at least 200 MB of free RAM per thread (>1GB per thread for large databases). Nonetheless, it is possible to run CrocoBLAST on large files even if less memory is available, but you will need to manually specify a small fragment size during job submission. Furthermore, if you need to analyze NGS data,the input and output files involved in such calculations can be quite large, and therefore you will need to have sufficient space on your hard disk. NCBI databases range from 10 MB to 500 GB (whole genomes). Depending on the type of sequencing experiment you ran, your input files may range from a few kB to hundreds of GB. If you don't specify any BLAST options, the size of the output file may be up to 1500 times the size of the input file. Nevertheless, in the typical use case, requesting relevant BLAST options (e.g., provide only the first 20 hits) will greatly reduce the size of the output file.