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=2. BLAST program= Depending on the nature of the query and reference sequences, there are several BLAST programs you may use within CrocoBLAST: *blastp - compares an amino acid query sequence against a protein sequence database *blastn - compares a nucleotide query sequence against a nucleotide sequence database *blastx - compares a nucleotide query sequence translated in all reading frames against a protein sequence database *tblastn - compares a protein query sequence against a nucleotide sequence database dynamically translated in all reading frames *tblastx - compares the six-frame translations of a nucleotide query sequence against the six-frame translations of a nucleotide sequence database Therefore, in order to run BLAST, you will need to indicate which BLAST program you intend to use.
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